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The wound exudates produced by the injection of two snake venom toxins into mice display contrasting proteomic profiles, illustrating the different mechanisms of tissue damage at play, say American researchers. [November 9, 2009] |
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Pulse chase experiments and 2-DE were combined in order to investigate how newly synthesized proteins are processed or modified to yield a functional yeast proteome. [November 2, 2009] |
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A panel of 4 proteins that can predict dry eye syndrome with an accuracy of 96% has been identified in human tears, with a subset that are associated with inflammation successfully classifying the clinical severity of the condition. [October 26, 2009] |
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Inhalation of mustard gas changes the proteomic profile of bronchoalveolar lavage fluid, say Iranian researchers, who examined long-term casualties exposed during the Iran-Iraq war. The altered profile will aid attempts to identify therapeutic targets for mustard gas-induced lung disease. [October 12, 2009] |
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In order to identify the proteins associated with Helicobacter pylori colonization in mice, 2-dimensional gel electrophoresis (2-DE) was used to analyze the membrane- and soluble-cellular proteins extracted from H. pylori strain 26695 and the mouse-passaged homolog 88-3887. [October 5, 2009] |
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This review assesses the impact of transcriptomics, proteomics and metabolomics on fungal plant pathology over the last decade and discusses their futures. Key examples highlighting the application of these technologies to fungal plant pathogens are also reviewed. [September 28, 2009] |
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The administration of recombinant human growth hormone to humans can be accomplished using a panel of six protein ions identified by MALDI MS or six peptide ions identified by LC/MS, say researchers in the UK. Their study combined proteomics with artificial neural networks in the first study of its type in biomarker discovery. [September 14, 2009] |
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A protocol was optimized for the extraction and an in-depth analysis of the soluble protein fraction of two nonaxenic toxin-producing cyanobacteria Cylindrospermopsis raciborskii (hepatotoxin-producing), and Raphidiopsis sp. (neurotoxin-producing), using 2D-PAGE. [September 7, 2009] |
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The seed storage proteome of the non-model species (Trifolium subterraneum), was characterised in mature dry seeds and during imbibition using two-dimensional electrophoresis coupled with tandem mass spectrometry. [August 24, 2009] |
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The allergen contents of different varieties of peanut have been compared by proteomics techniques, revealing the existence of hitherto unreported fragments of major allergens. Indonesian nuts were found to contain lower amounts of major allergens, which has implications for food production. [August 10, 2009] |
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Narrow-range peptide IEF was applied to plasma or pleural effusion prior to LC/MS/MS using IPG strips and free-flow electrophoresis. Data was compared with cell line data to evaluate the method performance in body fluids. [August 3, 2009] |
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The effects of equine drugs of abuse on the levels of proteins in horse plasma have been studied by scientists in the UK, leading to the discovery of candidate protein biomarkers for doping. These candidates were detected and measured using targeted analysis of their tryptic peptides. [July 27, 2009] |
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Obesity is associated with changes in the protein composition of low-density lipoprotein (LDL), as well as high levels of LDL cholesterol, according to scientists in Sweden. The transport protein transthyretin was found to be enriched in LDL from obese subjects and may be linked to an increased risk of cardiovascular disease. [July 13, 2009] |
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The proteome of a wine yeast was monitored by 2-D gel electrophoresis throughout alcoholic fermentation, revealing substantial changes in protein abundance which were not directly associated with changes at transcript level. [July 6, 2009] |
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A review of proteomics studies in the literature covering five species has revealed a common set of differentially expressed proteins, representing a set of regularly detected protein families. Many are linked to stress response, and their use as biomarkers of any other condition should be considered with caution. [June 22, 2009] |
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The metabolic pathways of rats subjected to acute and chronic stress varied in the same way but the changes were reversible once the stress was withdrawn. Chronic stress also induced behavioural and physiological signs of depression which were supported by changes in the levels of metabolites associated with depression. [June 8, 2009] |
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Proteomics techniques were used to find useful fibrosis markers in the sera of hepatitis C patients with different degrees of fibrosis, leading to new candidate biomarkers in line with fibrosis progression. [June 1, 2009] |
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A comparison of the proteome of matched synovial fluid and plasma samples from patients with juvenile idiopathic arthritis has revealed a set of proteins that differentiate the fluid from the plasma. They also segregate patient subgroups, allowing for clinical discrimination and flagging up patients for different treatment strategies. [May 25, 2009] |
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The venoms of four pit vipers have been characterised following preliminary fractionation by affinity binding to an anti-haemorrhagic protein derived from an opossum that is resistant to snake venom. The method separated metalloproteinases from the other protein classes, permitting more detailed study. [May 11, 2009] |
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A large-scale proteomic analysis of secreted proteins from a suspension of rice callus culture identified stress response proteins, metabolic proteins and factors involved in protein modification, with 25 novel rice-specific secreted proteins. [May 4, 2009] |
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