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Ion Chromatography Ezine
Charging down a pH gradient Charging down a pH gradient 
Because therapeutic antibodies vary in a way that synthetic drug compounds don't, two US scientists have developed a novel ion exchange chromatography method for determining antibody charge states. [November 9, 2009]

Ion chromatography tandem mass spectrometry for simultaneous confirmation and determination of indandione rodenticides in serum Ion chromatography tandem mass spectrometry for simultaneous confirmation and determination of indandione rodenticides in serum  [registered users only]
Chinese scientists have developed a simple ion chromatography-based method for simultaneously determining and confirming the indandione rodenticides in blood serum, for use in clinical diagnosis and forensic toxicology. [November 2, 2009]

Characterization of kiwifruit xyloglucan Characterization of kiwifruit xyloglucan 
Using various analytical techniques, including anion-exchange chromatography, an international team of scientists has purified and analysed the cell wall molecule known as xyloglucan in kiwi fruit. [October 26, 2009]

Halloween fright for fungal pathogens Halloween fright for fungal pathogens 
As if scaring away ghosts and ghouls at Halloween were not enough, it appears that pumpkins can also put the frighteners on disease-causing fungi. [October 12, 2009]

Mathematical modeling of elution curves for a protein mixture in ion exchange chromatography applied to high protein concentration Mathematical modeling of elution curves for a protein mixture in ion exchange chromatography applied to high protein concentration 
Chilean biochemical engineers have developed a model for simulating elution curves for the proteins alpha-lactalbumin, bovine serum albumin and conalbumin under different operational conditions. [October 5, 2009]

Characterization of monoclonal antibodies using polymeric cation exchange monoliths in combination with salt and pH gradients Characterization of monoclonal antibodies using polymeric cation exchange monoliths in combination with salt and pH gradients 
Using cation exchange polymethacrylate-based monolithic columns and imaged capillary electrophoresis, Australian and US researchers have characterised three different monoclonal antibody formulations of pharmaceutical interest. [September 28, 2009]

Taking on the jellies Taking on the jellies 
Jellyfish might not be too welcomed by those swimming in the sea, but ion chromatography has helped to reveal their potential benefit to those suffering from skin blemishes. [September 14, 2009]

Ionic determinants of exhaled breath condensate pH before and after exercise in adolescent athletes Ionic determinants of exhaled breath condensate pH before and after exercise in adolescent athletes  [registered users only]
Using ion chromatography, three US scientists have analysed the ionic composition of the exhaled breath condensate of adolescent athletes before and after exercise. [September 7, 2009]

Profiling the potential biomarkers for cell differentiation of pancreatic cancer using iTRAQ and 2-D LC-MS/MS Profiling the potential biomarkers for cell differentiation of pancreatic cancer using iTRAQ and 2-D LC-MS/MS  [registered users only]
Chinese medical researchers have used the iTRAQ-labeling technology and 2-D strong cation exchange/reversed phase liquid chromatography to profile the proteins secreted from pancreatic cancer cells. [August 24, 2009]

Sifting through stars Sifting through stars 
By finding a way to sift through more DNA strands than there are stars in the observable universe, US scientists came up with the most sensitive method yet for separating and purifying different types of carbon nanotube. [August 10, 2009]

Chemical composition and mass closure for aerosols during the 2005 dry season at a rural site in Morogoro, Tanzania Chemical composition and mass closure for aerosols during the 2005 dry season at a rural site in Morogoro, Tanzania  [registered users only]
Using a range of analytical techniques, including ion chromatography, Belgian scientists have determined the chemical composition of the atmospheric particulate matter collected at a rural site in Morogoro, Tanzania. [August 3, 2009]

Characterisation of flucloxacillin and 5-hydroxymethyl flucloxacillin haptenated HSA <i>in vitro</i> and <i>in vivo</i> Characterisation of flucloxacillin and 5-hydroxymethyl flucloxacillin haptenated HSA in vitro and in vivo  [registered users only]
Using cation exchange, affinity and reversed phase chromatography, British researchers have investigated what causes adverse reactions to the synthetic antibiotic flucloxacillin, which is used to treat Staphylococcal infections. [July 27, 2009]

Sweet test for PKU Sweet test for PKU 
By using an anion-exchange chromatography column designed for separating sugars to detect an amino acid, South Korean researchers have come up with a novel way to diagnose a serious genetic disorder. [July 13, 2009]

Separation with zwitterionic hydrophilic interaction LC improves protein identification by MALDI-based proteomic analysis Separation with zwitterionic hydrophilic interaction LC improves protein identification by MALDI-based proteomic analysis  [registered users only]
Japanese scientists have investigated using zwitterionic hydrophilic interaction liquid chromatography to separate the peptides produced by digesting human serum albumin, which is the most abundant protein in human blood plasma. [July 6, 2009]

Pilot-scale ion-exchange centrifugal partition chromatography: Purification of sinalbin from white mustard seeds Pilot-scale ion-exchange centrifugal partition chromatography: Purification of sinalbin from white mustard seeds 
Using strong ion-exchange centrifugal partition chromatography, French scientists managed to purify the glucosinolate sinalbin on a multigram scale from a crude aqueous extract of white mustard seeds. [June 22, 2009]

The acetonitrile shortage: Is reversed HILIC with water an alternative for the analysis of highly polar ionizable solutes? The acetonitrile shortage: Is reversed HILIC with water an alternative for the analysis of highly polar ionizable solutes?  [registered users only]
An LC/MS procedure was used to measure levels of cocaine and its metabolites benzoylecgonine and ecgonine methyl ester in wastewater samples from 41 wastewater treatment plants in Belgium. [June 19, 2009]

Honey, honey Honey, honey 
Anion exchange chromatography seems to have a taste for honey, being able to distinguish pure honey from honey adulterated with corn syrup at concentrations as low as 1%. [June 8, 2009]

Arsenic speciation in cattail using chromatography and mass spectrometry Arsenic speciation in cattail using chromatography and mass spectrometry  [registered users only]
Canadian scientists have used both cation and anion exchange chromatography to determine arsenic species present in cattail, a type of wetland plant that is widely used as a traditional food and medicinal ingredient by indigenous people. [June 1, 2009]

Chromatographic isolation and characterization of a novel peroxidase from large lima legumes Chromatographic isolation and characterization of a novel peroxidase from large lima legumes  [registered users only]
Chinese and US scientists have developed a multi-step chromatography process, including ion exchange and affinity chromatography, for isolating a novel peroxidase with antifungal activity from large lima beans. [May 25, 2009]

Soaking up the heat Soaking up the heat 
A novel ion exchange stationary phase strongly binds whole proteins at 50°C but then releases them at 20°C, offering an inexpensive and environmentally-friendly way to isolate and purify proteins. [May 11, 2009]

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